Laurent
NUSSAUME
CEA Research Director
BIAM, Saint Paul lez Durance, FRANCE
Our research focuses on Phosphate: a main mineral macro-nutrient for plants. We study its uptake and associated signaling pathways. We combine genetics, molecular biology, plant physiology, radio-imaging and cellular biology. For such a purpose, we have developed state-of-the-art technologies such as live cell transcriptomics imaging using MS2/MCP system coupled with microfluidics (Hani et al., 2021) and a novel reporter system facilitating dynamic study of protein/protein interactions in plants (under review).
We are currently
I
Identifying multiple A.thaliana mutations affecting Phosphate signal transduction
II
Creating a novel generation of ion sensors to follow internal nutritional status with single-cell resolution
III
Coupling the MS2/MCP system with Anchor technology (in collaboration with F. Pontvianne) to study intra- and inter-cell transcriptional variability during Phosphate starvation (AGILE project). This system will also be used to monitor the physiological impact of the mutations identified (i)
IV
Develop a dynamic system offering access to protein-protein interaction dynamics to study interactions of regulatory proteins taking place during Phosphate signal transduction (AGILE project)
Five Main Publications
Live single-cell transcriptional dynamics via RNA labelling during the phosphate response in plants
Hani, S. et al. Nat Plants 7, 1050-1064 (2021).
Visualizing plant intracellular inorganic orthophosphate distribution
Guo, M. et al. Nat Plants 10, 315-326 (2024).
A novel role for the root cap in phosphate uptake and homeostasis
Kanno, S. et al. eLife 5, e14577 (2016).
Cracking the code of plant central phosphate signaling
Jia, X. Q., Wang, L., Nussaume, L. & Yi, K. K. Trends in plant science 28, 267-270 (2023).
Arabidopsis thaliana high-affinity phosphate transporters exhibit multiple levels of posttranslational regulation
Bayle, V. et al. Plant Cell 23, 1523-1535 (2011).