Frédéric
PONTVIANNE
CNRS Research Director
ORCID link : 0000-0002-2913-4104
Website links : Research and team CODED
iGRED, Clermont-Ferrand, France
Chromatin organization and dynamic in plant
My research group focuses on developing tools that can be used in microscopy to study the position and expression of genes with a single-cell and allelic resolution. To this end, we have adapted the ANCHOR system (NeoVirTech®) in plants to monitor the position of a single locus in the genome (Meschichi et al., 2021).
We are currently
I
Creating a collection of A. thaliana lines with a variable genomic position of the ANCHOR system to make them visible by confocal microscopy directly in planta (ANR Dyscord)
II
Developing innovative microscopy methodologies to characterise gene expression with allelic resolution in order to explore, in particular, intra- and inter-cell transcriptional variability, or the phenomenon of allelic clustering, especially during abiotic stress (AGILE project)
III
Using our microscopy-based methods to understand the interaction between Agrobacterium tumefaciens and Arabidopsis thaliana via the monitoring the T-DNA once injected into root cells by (ANR Mobile-DNA)
IV
Developing tools to monitor Transposable Element activity directly in planta.
Five Main Publications
Role of methylation and siRNA on differential allelic expression in a hybrid of distantly related citrus species bioRxiv (2025)
Diop K, Gibert A, Llauro C, Froelicher Y, Hufnagel B, Picault N, Pontvianne F#. (2025) 07.25.666733; doi: 10.1101/2025.07.25.666733
Extrachromosomal circular DNA and structural variants highlight genome instability in Arabidopsis epigenetic mutants
Zhang P, Mbodj A, Soundiramourtty A, Llauro C, Ghesquière A, Ingouff M, Slotkin RK, Pontvianne F, Catoni M, Mirouze M. Nat Commun. (2023) Aug 28;14(1):5236. doi: 10.1038/s41467-023-41023-0.
ANCHOR: A Technical Approach to Monitor Single-Copy Locus Localization in Planta
Meschichi A, Ingouff M, Picart C, Mirouze M, Desset S, Gallardo F, Bystricky K, Picault N, Rosa S, Pontvianne F#. Front. Plant Sci., (2021) 12:677849. https://doi.org/10.3389/fpls.2021.677849.
Large tandem duplications affect gene expression, 3D organization, and plant–pathogen response
Picart-Picolo A, Grob S, Picault N, Franek M, Llauro C, Halter T, Maier TR, Jobet E, Descombin J, Zhang P, Paramasivan V, Baum TJ, Navarro L, Dvoráková M, Mirouze M, Pontvianne F#. Genome Res. (2020) Nov;30(11):1583-1592. doi:10.1101/gr.261586.120
Identification of Nucleolus-Associated Chromatin Domains Reveals a Role for the Nucleolus in 3D Organization of the A. thaliana Genome
Pontvianne F, Carpentier MC, Durut N, Pavlištová V, Jaške K, Schořová Š, Parrinello H, Rohmer M, Pikaard CS, Fojtová M, Fajkus J, Sáez-Vásquez J. Cell Rep. (2016) Aug 9;16(6):1574-1587. doi: 10.1016/j.celrep.2016.07.016.